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Tuesday, July 14, 2020 | History

2 edition of Regulation and characterization of an extracellular ribonuclease from Neurospora crassa found in the catalog.

Regulation and characterization of an extracellular ribonuclease from Neurospora crassa

Richard Allen Lindberg

Regulation and characterization of an extracellular ribonuclease from Neurospora crassa

by Richard Allen Lindberg

  • 59 Want to read
  • 36 Currently reading

Published .
Written in English

    Subjects:
  • Enzymes -- Analysis.,
  • Neurospora crassa.

  • Edition Notes

    Statementby Richard Allen Lindberg.
    The Physical Object
    Paginationviii, 43 leaves, bound :
    Number of Pages43
    ID Numbers
    Open LibraryOL16565680M

    These include, for example, the genes hex ‐1 of Neurospora crassa and pacC of Aspergillus nidulans, which respond to extracellular phosphate changes and ambient pH, respectively []. The filamentous fungus N. crassa is a heterothallic, free‐living, eukaryotic microorganism that is widespread in nature and is clearly visible due to its. In Neurospora crassa, three transport systems have been described based on the analysis of the kinetics of amino acid uptake and the patterns of competitive inhibition between amino acids. The Gap1 homolog in Neurospora crassa is encoded by the PMG locus, which can transport all .

      The pH-signaling pathway controls accumulation of the reserve carbohydrate glycogen and trehalose in Neurospora crassa. We previously identified the PAC-3 transcription factor as a putative regulator of glycogen metabolism [], and later we showed that PAC-3 is required for proper glycogen accumulation by down-regulating the expression of the gene encoding glycogen synthase . The filamentous fungi display remarkable and diverse metabolic pathways and show great versatility in the utilization of sources of carbon, nitrogen, phosphorus, sulfur, and other metabolites and in acquiring essential elements, e.g., iron and potassium. This chapter emphasizes practical aspects, methods, and simple assays for investigating fungal physiology and metabolism and introduces some.

    P-starved plants scavenge inorganic phosphate (Pi) by developing elevated rates of Pi uptake, synthesizing extracellular phosphatases, and secreting organic acids. To elucidate mechanisms controlling these acclimation responses in photosynthetic organisms, we characterized the responses of the green alga Chlamydomonas reinhardtii to P starvation and developed screens for isolating mutants. The relative concentrations of secreted proteins in liquid cultures of Neurospora crassa differ in constant darkness compared to constant light (lx). Light reduces the concentrations of some polypeptides markedly and increases the concentrations of protein species of 67, 40, 18 and 13 kDa. The “blind” wc-2 mutant of Neurospora does not show light dependent differences in amounts of.


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Regulation and characterization of an extracellular ribonuclease from Neurospora crassa by Richard Allen Lindberg Download PDF EPUB FB2

Regulation ofaNeurospora crassa Extracellular RNaseby Phosphorus, Nitrogen, and CarbonDerepressions RICHARDA. LINDBERG*ANDHARVEYDRUCKER Biology andChemistry Department, Pacific NorthwestLaboratory, Richland, Washington Received 2 August /Accepted 4 November Anewextracellular RNase,designated N4, wasdetectedin culture.

A new extracellular RNase, designated N4, was detected in culture filtrates from Neurospora crassa and its regulation was studied.

Limitation of a nutrient obtainable from RNA alone was not sufficient to cause enzyme derepression. The addition of RNA Cited by: 9. Lindberg RA, Drucker H. Regulation of a Neurospora crassa extracellular RNase by phosphorus, nitrogen, and carbon derepressions. J Bacteriol.

Feb; (2)– [PMC free article] Lindberg RA, Eirich LD, Price JS, Wolfinbarger L, Jr, Drucker H. Alkaline protease from Neurospora crassa. Purification and partial by: 7.

Cohen BL. Regulation of intracellular and extracellular neutral and alkaline proteases in Aspergillus nidulans. J Gen Microbiol. Dec; 79 (2)– Drucker H. Regulation of exocellular proteases in Neurospora crassa: induction and repression of enzyme synthesis.

J Bacteriol. Jun; (3)– [PMC free article]Cited by:   Takai N, Uchida T, Egami F. Purification and properties of ribonuclease N1, an extracellular ribonuclease of Neurospora crassa.

Biochim Biophys Acta. Oct 17; (1)– [Google Scholar] Turner JR, Matchett WH. Alteration of tryptophan-mediated regulation in Neurospora crassa by indoleglycerol phosphate. J Bacteriol. Cohen BL, Morris JE, Drucker H. Regulation of two extracellular proteases of Neurospora crassa by induction and by carbon-nitrogen and sulfur-metabolite repression.

Arch Biochem Biophys. Jul; (1)– Drucker H. Regulation of exocellular proteases in Neurospora crassa: induction and repression of enzyme synthesis. J Bacteriol. Extracellular RNase N4 from Neurospora crassa is derepressible by limitation of any of the three nutrient elements obtainable from RNA.

We have purified and characterized the enzyme from cultures. Lerch K, Longoni C, Jordi E. Primary structure of tyrosinase from Neurospora crassa.

Purification and amino acid sequence of the cyanogen bromide fragments. J Biol Chem. Jun 10; (11)– Lindberg RA, Drucker H. Regulation of a Neurospora crassa extracellular RNase by phosphorus, nitrogen, and carbon derepressions.

J Bacteriol. A strain of Neurospora crassa defective in amino acid transport can utilize a variety of amino acids for growth when readily metabolizable nitrogen is limiting. Growth is accompanied by the production of an extracellular deaminase that converts the amino acid to its respective keto acid plus equimolar quantities of utilizable nitrogen in the.

The production of pectinase was studied in Neurospora crassa, using the hyperproducer mutant exo-1, which synthesized and secreted five to six times more enzyme than the wild-type.

A new species of orthophosphate repressible extracellular 5′-nucleotidase (5′-ribonucleotide phosphohydrolase, EC ) was found to be released into mycelial culture media when a wild type strain of Neurospora crassa was grown on limiting amounts of phosphate.

The production of 5′-nucleotidase and extracellular acid and alkaline phosphatase was inhibited by the addition of. Regulation of a Neurospora crassa extracellular RNase by phosphorus, nitrogen, and carbon derepressions.

Lindberg RA, Drucker H. J Bacteriol, (2), 01 Feb Cited by: 9 articles | PMID: | PMCID: PMC Free to read. At least four species of nucleases (nuclease N1, N2, N3 and N4) and one ribonuclease (ribonuclease N3) were detected in extract of wild type mycelia grown in high phosphate media by gel filtration of 0–65% ammonium sulfate precipitate through Sephadex G Nuclease N4 eluted the first is a latent nuclease, the activity of which is not detectable within a week after preparation of the.

Lindberg RA, Drucker H. Regulation of a Neurospora crassa extracellular RNase by phosphorus, nitrogen, and carbon derepressions. J Bacteriol. Feb; (2)– [Europe PMC free article] [Google Scholar] Lindberg RA, Eirich LD, Price JS, Wolfinbarger L, Jr, Drucker H. Alkaline protease from Neurospora crassa.

A putative extracellular RNase (SNOG_) represented in spot E20 suggests that extracellular nucleic acid metabolism may be subjected to regulation by heterotrimeric G protein signalling. nodorum is a necrotrophic pathogen and consequently the degradation of host tissue would result in the release of nucleic acids.

An extracellular lipase from the dimorphic yeast The pH optimum of and a temperature optimum of 30 °C are similar to that of the Neurospora crassa purification and characterization of an extracellular lipase from Aureobasidium pullulans HN with potential application for the hydrolysis of edible oils, Biochemical.

A simple and rapid procedure for isolation of a highly purified ribonuclease from the culture filtrate of Trichoderma harzianum was described.

The sch. The abilities of purine- and pyrimidinerequiring mutants to produce six orthophosphate repressible extracellular enzymes, alkaline phosphatase, 5′-nucleotidase, acid phosphatase, two nucleases and ribonuclease N1 were examined by culturing these mutants in low and high phosphate media containing nucleotide or nucleoside.

All the purine requiring mutants produced significantly reduced amounts. Molecular Cloning and Characterization ofthe cys-3 Regulatory GeneofNeurospora crassa and an extracellular protease (9, 10, 13, of sulfur regulation at the molecular level is lacking. For. Isolation and Characterization of the adenylate cyclase structural gene of Neurospora crassa.

Article (PDF Available) in The Japanese Journal of Genetics 66(3) July with 15 Reads. Allgaier et al. () evaluated promoters of the N. crassa genes cfp, ccg-1, acu-5, cpc-2, and enolase for the expression of N. crassa ribonuclease N 1 (RNase N 1) and sequence-optimized bovine.Regulation and characterization of Thermobifida Wang, Yan Yang, Yisong Liu, Duoduo Zhang, Shijie Guo, Qian Liu, Hao Fang, Yahong Wei, The Model Filamentous Fungus Neurospora crassa: Progress Toward a Xingliang Pan, Junxiu Hou, Chao Ran, Zhigang Zhou, Improving extracellular production of Serratia marcescens lytic.Biofuels derived from lignocellulosic biomass are a viable alternative to fossil fuels required for transportation.

Following plant biomass pretreatment, the furan derivative furfural is present at concentrations which are inhibitory to yeasts. Detoxification of furfural is thus important for efficient fermentation.

Here, we searched for new genetic attributes in the fungus Neurospora crassa.